Lab Reports

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FREIE UNIVERSITY BERLIN (FU BERLIN) Hautklinik und Poliklinik Im Rudolf-Virchow-Krankenhaus (FB 3, WE 15) Direktor: Prof. Dr. med. G Stüttgen Freie Universität Berlin, FB 3, WE 15, 1000 Berlin 65, Augustenburger Platz 1 Tel. 4505-1 Extn. 2348 Since June 1981, Mink Oil Pure has been routinely examined within the scope of Epicutaneous tests and compatibility tests as well as in application to normal test persons and patients with sensitive skin. It was determined that Mink Oil revealed no incompatibility in any of the aforementioned test procedures and applications. Even patients displaying extreme sensitivity toward basic ingredients contained in ointments used for cosmetic purposes and in dermatological therapy showed no reaction to Mink Oil Pure. In further tests, the skin reaction in the areas to which Mink Oil had been applied was tested with regard to intensive exposure to ultraviolet B and A. An undesired phototoxic reaction was not determined. These tests were conducted routinely, and the analysis of the Mink Oil revealed no substances capable of a phototoxic reaction. Examinations of the skin surface fat after application of Mink Oil Pure revealed that Mink Oil Pure penetrates the deeper layers of skin. Within ½ hour, Mink Oil diffuses into the lower layers of the skin, thus preventing an undesired, long lasting greasing effect starting from the skin surface. Mink Oil Pure is undoubtedly a substance patients like to use for skin care. The cosmetic characteristics of Mink Oil Pure skin care have an effect on both dry and normal skin. Of particular value from the dermatological viewpoint is the statement that an incompatibility reaction of skin irritation was not revealed, even under particular stress in a clinical experimental sense as well as under normal exposure. This statement pertains to the aforementioned pure mink oil, which contained no additives. A total of 238 patients of a dermatology clinic were tested in area of unchanged skin. In addition, mink oil was used for the after-treatment of exsiccated skin following recent dermatosi whereby a total of 18 patients showing an incompatibility with Lanolin tolerated Mink Oil Pure without any reaction. The same was found with 4 patients displaying an incompatibility with Lanette. Thus, Mink Oil Pure appears to be a raw material...

Study made in collaboration with Services of Laboratory of Chemistry and Bacteriology of City of Gent, department of Belgium Ministry of Health. ADVANTAGES OF MINK OIL AS INGREDIENT IN COSMETIC PRODUCTS SPREADABILITY Mink Oil has a spreading coefficient: on water of approx. 2 times that of IPM (Isopropyl Myristate), and 3 times that of European Sesbellot?Mink Oil of identical viscosity. OXIDATION STABILITYMink Oil lays much higher than most of other animal oils. This not withstanding characteristics due to the nature of Mink Oil: containing 70-80% unsaturated fats and having an iodine value of 80 to 95.Its oxidation stability was confirmed by A.O.M. test (active oxygen method) which consists in heating oil at 98.7°C while bubbling a constant stream of air through it. Periodically peroxide Values(PV) are determined until PV=20. This PV is expressed as milli equivalents (Meq) of Peroxide per Kg of oil. A.O.M. numbers of hours of accelerated oxidative treatment of the oil. OIL PV = 20 eached in hours Lard 4 Cottonseed oil 5 Safflower oil 10 – 12 Corn oil 12 – 25 Peanut Oil 20 Wheat Germs Oil 25 – 30 Sesame Oil 36 Mink Oil 40 – 50 A very effective, natural anti-oxidizer (blend of alpha, beta, gamma tocopherol) is added when melting the mink fat. This allows keeping the Mink Oil smelling perfectly fresh for 12 to 18 months when stored in airtight sealed and at temperature of 14 to 18°C. This storage durability time is 24 months and more for Mink Oil in sealed glass bottles under carton cover (retail packaging).The F.F.A. (free fatty acids) coefficient calculated on Mink Oil taken out of normal daily production shows 0.05 to 0.1%(Oleic acid). UV ABSORPTION CAPACITY UV absorption capacity of Mink Oil: another of its characteristics. It lays much higher than any other commonly used animal ingredients. Even higher than oils such as sesame, avocado, since long times used for sunscreen formulations. It shows distinct UV absorption peak sat 2070-2080µm. In operative concentration ranges of 5 to 10% Mink Oil, it releases a thin continuous film coating the body evenly due to its favorable Mink Oil used in sun protective formulations completed with special UV sunscreen a most wonderful products: perfectly protecting & caring. EMULSIFICATION Emulsification of...

Study made in collaboration with Services of Laboratory of Chemistry and Bacteriology of City of Gent, department of Belgium Ministry of Health. CHEMICAL ASPECTS OF MINK OIL [COMPOSITION] C-12:0 Dodecane acid(Lauric Acid) 0.30% C-14:0 Tetradecane acid(Myristic Acid) 3.50% C-15:0 Pentadecane acid 0.20% C-16:0 Hexadecane Acid(Palmitic Acid) 16.00% C-16:1 Hexadecane Acid(Palmitoleic Acid) 15.00% C-17:0 Heptadecane Acid(Margarin Acid) 0.20% C-17:1 Heptadecane Acid 0.50% C-18:0 Octadecane Acid(Stearic Acid) 3.00% C-18:1 Octadecane Acid(Oleic Acid) 39.5% C-18:2 Octadecatriene Acid(Linoleic Acid) 16.00% C-18:3 Octadecatriene Acid(Linolenic Acid) 1.00% C-20:0 Eicosane Acid(Arachic Acid) 0.20% C-20:1 Eicosene Acid 1.50% C-20:4 Eicosatetraene Acid 0.40% C-20:5 Eicosapentaene Acid 0.50% C-22:0 Docosane Acid traces C-22:1 Docosene Acid 0.20% C-22:5 Docopentaene Acid 0.40% C-22:6 Docohexaene Acid 0.60% C-24:0 Tetracosane Acid 0.10% Content of unsaturated fatty acids more than 75% Content of polysaturated fatty acids less than 20% Free fatty acids < 0.1 Iodine Value 84-89 Refraction Index 1.4670-1.4706 Saponification Number 195-200 Mink Oil contains 14-18% Palmitoleic Acid: C-16 unsaturated found in no other commonly used cosmetic ingredient. It is the closest to human sebum. Best tolerated and most beneficial for the skin. Mink Oil contains 70% to 80% Unsaturated Fats: It tends to rub directly into the skin, where it penetrates deeply, leaving a velvety rich texture. However, leaving a non greasy nor sticky felling behind. For these reasons, one can say Mink Oil is completely different to almost all other natural oils used in...

Study made in collaboration with Services of Laboratory of Chemistry and Bacteriology of City of Gent, department of Belgium Ministry of Health. COMPARISON STUDY BETWEEN DIFFERENT OILS ON BEHALF THEIR PENETRATION POWER INTO HUMAN SKIN For each experimented oil the scale results give the average necessary time for penetrating into the human skin and also their type typical digressions. Out of the gathered results, we now can draw several conclusions. The studied oils can be grouped in 3 categories according to their penetration through the skin. Those with highest penetration power. ( in digressive line) European Sesbellot™ Mink Oil – Linseed Oil – Cartharme Oil – Avocado Oil Those with medium penetration power; Walnut Oil – Tuna Oil – Rye Germs Oil – Wheatgerm Oil – Carob Germs Oil – Maize Oil – Codliver Oil – Soya Oil – Sweet Almond Oil – Hazelnuts Oil. Those with low penetration power Olive Oil – Colzaseed Oil-Rice Germs Oil. [SCALE IN DEGRESSIVE LINE] Type of Oil Average penetration time Typical Digressions Mink Oil 19.0 2.5 mm Line seed Oil 20.0 2.4 mm Cathame Oil 21.0 1 mm Avocado pear Oil 23.0 3.4 mm SoyabeansOil-lecithinpoor 23.7 2.5 mm Walnut Oil 26.7 2.2 mm Rye germs Oil 28.5 2.3 mm Tuna fish Oil 28.5 4.5 mm Carob germ Oil 29.0 4.8 mm WheatgermOillecithinpoor 29.4 3 mm Maize germ Oil 32.0 4.8 mm Codliver Oil 33.6 4.5 mm Hazelnut Oil 39.0 1 mm Soya beans Oil-normal 41.6 1.8 mm Sweet Almonds Oil 44.0 5 mm Wheat Germ Oil-normal 48.0 5 mm Olive Oil 54.0 4 mm Rice Germ Oil 70 mm Colzaseed Oil 80 mm 4. The Pharmacist naturally will tend to interpret these biological activity differences according to chemical or physical characteristic differences that mainly are. A. Viscosity B. Iodine Incidence (non saturated fatty acids) C. % of unsaponifiable material D. % of lecithin E. Length of carbon chain of constituent fatty acids. 1. VISCOSITY The negative influence of liquid viscosity on penetration power through the skin has since long has been noted by many authors. : Brown & Scott 1934-Valette, Cavier & Savelle 1954- Hadgraft 1961. In fact, some of our results meet their assertions: it is according to their very low...